Research can be frustrating and stressful. That is this week's lesson. I conducted my first experiment on my own (with much hep from my PhD student) and of course my RNA purities were too low to run RT-PCR data. So now I have to wait until next week's macrophages are ready and try again. Apparently this happens to everyone on their first try. The problem is that the lab I am working in is getting ready to publish and the PI is very results oriented. So there is not much time for screw-ups like these. I ended the week feeling frustrated and useless; I felt like I was bringing the lab down.
After a relaxing, chill weekend with some errands, a few drinks, and some quality sleep I have a new attitude. I will try my best since everyone, including the PI, knows I am brand new. I can only do all that I can. I will not beat myself up for not getting results, and in the end everything will be fine. Perhaps I don't have a career in research or will not get published, but at least I tried something new and put my best foot forward.
My first Monday with my new attitude has gone well. The insulin tolerance testing on the new study mice went well this morning.